JACS
Rachel K. O’Reilly
http://pubs.acs.org/doi/abs/10.1021/ja3105494
Dithiomaleimides (DTMs) with alkyl substituents are shown to be a novel class of highly emissive fluorophores. Variable solubility and further functionalization can easily be tailored through the choice of N and S substituents. Inclusion of a DTM unit into a ROP/RAFT initiator or insertion into the disulfide bond of salmon calcitonin (sCT) demonstrates the utility for fluorescent labeling of polymers and proteins. Simultaneous PEGylation and fluorescent labeling of sCT is also demonstrated, using the DTM unit as both a linker and a fluorophore.
Rachel K. O’Reilly
http://pubs.acs.org/doi/abs/10.1021/ja3105494
Dithiomaleimides (DTMs) with alkyl substituents are shown to be a novel class of highly emissive fluorophores. Variable solubility and further functionalization can easily be tailored through the choice of N and S substituents. Inclusion of a DTM unit into a ROP/RAFT initiator or insertion into the disulfide bond of salmon calcitonin (sCT) demonstrates the utility for fluorescent labeling of polymers and proteins. Simultaneous PEGylation and fluorescent labeling of sCT is also demonstrated, using the DTM unit as both a linker and a fluorophore.
Protein, antibodies, peptides, nucleic acids, ligands, synthetic oligonucleotides and other biomolecules, labeling with fluorescent molecules that allows the sensing and visualizing of protein dynamics, Fluorescent labeling
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