Thiol-Selective Fluorogenic Probes for Labeling and Release

Vu Hong, Alexander A. Kislukhin, and M. G. Finn Journal of the American Chemical Society 2009 131 (29), 9986-9994

http://pubs.acs.org/doi/abs/10.1021/ja809345d

This article outlines a technique that utilizes thiol-ene chemistry to link peptides and other molecules together. When the thiol reacts with the oxonorbornadiene moiety, it activates a fluorescent dye that is attached. These adducts decompose readily via reverse Diels-Alder.