Bozhi Tian, Jia Liu, Tal Dvir, Lihua Jin, Jonathan Tsui, Quan Qing, Zhigang Suo, Robert Langer, Daniel Kohane & Charles M. Lieber
The authors developed a nanoelectronic scaffold that can be incorporated into 3D biomimetic ECM allowing for electrical probing of the bio-microenvironment. First, nanoelectronic scaffold is formed either by lithography or self-assembly in 2D on a nickel layer, which is then removed, allowing the scaffold to rearrange. The metal scaffold is designed to have high porosity and flexibility. Gel casting and electrospinning were used to form gels around the scaffold. SEM images show that the scaffold and the gels are fully entagled, but the electronic properties of the scaffold remained unchanged.
Cardiomyocyte cells were cultured onto the gel matrix. The scaffold could detect changes in conductance which corresponded to cellular contraction. When treated with norepinephrine, the increase contraction frequency could be detected.
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