Digital photography for the analysis of fluorescence responses

himon Schwaebel, Oliver Trapp and Uwe H. F. Bunz
http://pubs.rsc.org/en/content/articlepdf/2013/sc/c2sc21412a

 The color change in pyridine substituted cruciform fluorophores (XF) in the presence of different carboxylci acids was evaluated using digital photography to address whether extraction of color from digital photography was a suitable, reproducible equivalent to spectrometers in obtaining quantitative information. Errors in reproducibility in pictures of fluorescent samples can arise from the excitation lamp used, sample positioning, exposure time, and white balance. The authors suggested taking the RGD values from a photograph and converting them into CIE LUV standardized color space, which does not contain brightness information.  One strong recommendation was to use a fixed white blance and slightly underexpose the fluorescent sample.