http://pubs.acs.org/doi/full/10.1021/bm301789d#
K. C. Koehler, K. S. Anseth, and C. N. Bowman
Jeremiah did a brief post on this paper, but I thought a slightly more in-depth post would be useful too.
In this paper, the authors explore the use of the Diels-Alder/retro-DA reaction as a "click" type method for tethering and releasing something from a hydrogel. They use a tetra-functional thiol and maleimide PEG stars to form the hydrogel, using a stoichiometric imbalance resulting in an excess of maleimide. Unreacted maleimides undergo a Diels-Alder reaction with a fluorescently labeled furan-RGDS peptide. They then examine the release profile of the fluorescent group as function of temperature and availability of reaction sites, finding a (not surprising) strong temperature dependence for release.
K. C. Koehler, K. S. Anseth, and C. N. Bowman
Jeremiah did a brief post on this paper, but I thought a slightly more in-depth post would be useful too.
In this paper, the authors explore the use of the Diels-Alder/retro-DA reaction as a "click" type method for tethering and releasing something from a hydrogel. They use a tetra-functional thiol and maleimide PEG stars to form the hydrogel, using a stoichiometric imbalance resulting in an excess of maleimide. Unreacted maleimides undergo a Diels-Alder reaction with a fluorescently labeled furan-RGDS peptide. They then examine the release profile of the fluorescent group as function of temperature and availability of reaction sites, finding a (not surprising) strong temperature dependence for release.
No comments:
Post a Comment