Naohiro Kameta, Mitsutoshi Masuda, and Toshimi Shimizu, ACS Nano Article ASAP
In this paper, the authors create self-assembled nanotube hydrogels that
are pH responsive. They were able to encapsulate denatured proteins
inside the nanotubes, using denatured GFP as a model system. By reducing
the concentration of denatured GFP and the concentration of the
denaturing agent, they saw an increase in folded GFP, evidenced by
fluorescence within the hydrogel. Folded protein remained inside the gel
due to electrostatic interactions, but could be removed (properly
folded) under basic conditions.
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