Sunday, June 10, 2012

Soft Nanotube Hydrogels Functioning as Artificial Chaperones

Naohiro Kameta, Mitsutoshi Masuda, and Toshimi Shimizu, ACS Nano Article ASAP

In this paper, the authors create self-assembled nanotube hydrogels that are pH responsive. They were able to encapsulate denatured proteins inside the nanotubes, using denatured GFP as a model system. By reducing the concentration of denatured GFP and the concentration of the denaturing agent, they saw an increase in folded GFP, evidenced by fluorescence within the hydrogel. Folded protein remained inside the gel due to electrostatic interactions, but could be removed (properly folded) under basic conditions.
Abstract Image

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