Bozhi Tian, Jia Liu, Tal Dvir, Lihua Jin, Jonathan Tsui, Quan Qing,
Zhigang Suo, Robert Langer, Daniel Kohane & Charles M. Lieber
The authors developed a nanoelectronic scaffold that can be incorporated
into 3D biomimetic ECM allowing for electrical probing of the
bio-microenvironment. First, nanoelectronic scaffold is formed either by
lithography or self-assembly in 2D on a nickel layer, which is then
removed, allowing the scaffold to rearrange. The metal scaffold is
designed to have high porosity and flexibility. Gel casting and
electrospinning were used to form gels around the scaffold. SEM images
show that the scaffold and the gels are fully entagled, but the
electronic properties of the scaffold remained unchanged.
Cardiomyocyte cells were cultured onto the gel matrix. The scaffold
could detect changes in conductance which corresponded to cellular
contraction. When treated with norepinephrine, the increase contraction
frequency could be detected.