Thursday, February 7, 2013

Drug delivery, cell surface

Nanoparticle Adhesion to the Cell Membrane and Its Effect on Nanoparticle Uptake Efficiency
Anna Lesniak,‡,† Anna Salvati,‡,† Maria J. Santos-Martinez,§,∥,⊥ Marek W. Radomski,§,⊥ Kenneth A. Dawson,‡,* and Christoffer Åberg‡,*

This article describes adsorption of PS-based NPs to cell surfaces and the subsequent internalization.  The authors find "that the adsorption of proteins on the nanoparticle surface strongly reduces nanoparticle adhesion in comparison to what is observed for the bare material. Nanoparticle uptake is described as a two-step process, where the nanoparticles initially adhere to the cell membrane and subsequently are internalized by the cells via energy- dependent pathways. The lowered adhesion in the presence of proteins thereby causes a concomitant decrease in nanoparticle uptake efficiency. The presence of a biomolecular corona may confer specific interactions between the nanoparticle-corona complex and the cell surface including triggering of regulated cell uptake. An important effect of the corona is, however, a reduction in the purely unspecific interactions between the bare material and the cell membrane, which in itself disregarding specific interactions, causes a decrease in cellular uptake." 

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