Thursday, February 7, 2013

MRI contrast agent: a different approach

Transforming Thymidine into a Magnetic Resonance Imaging Probe for Monitoring Gene Expression
Amnon Bar-Shir,†,‡ Guanshu Liu,†,§ Yajie Liang,†,‡ Nirbhay N. Yadav,†,§ Michael T. McMahon,†,§ Piotr Walczak,†,‡ Sridhar Nimmagadda,∥ Martin G. Pomper,∥ Keri A. Tallman,⊥ Marc M. Greenberg,⊥ Peter C.M. van Zijl,†,§ Jeff W.M. Bulte,†,‡,§,#,∇,○ and Assaf A. Gilad*,†,‡,§ 

This paper describes an NMR-timescale proton exchange of imino (in a thymidine analogue) and water protons, leading to chemical exchange saturation transfer (CEST) contrast.  This mechanism relies on phosphorylation-based mechanism for the localization of the thymidine analogue in cells that express HSV1-tk genes.

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