Sunday, July 8, 2012

Site-Specific Attachment of a Protein to a Carbon Nanotube End without Loss of Protein Function

Bioconjugate Chemistry

The authors attached calmodulin to the ends of carbon nanotubes and demonstrated that the binding capacity of calmodulin remained unchanged. Protein attachment was done via a site-specific mutation of Tyr80 to azido-tyrosine, which was then coupled to oxidized CNTs using a Staudinger-Bertozzi ligation. Calmodulin binding was demonstrated using an ECFP-calmodulin-binding peptide fusion protein. Treatment with ECFP-CBP showed binding only on the ends of the CNTs.

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