In vivo temperature controlled ultrasound-mediated intracellular delivery of cell-impermeable compounds

Anna Yudina, Matthieu Lepetit-Coiffé, Mariska De Smet, Sander Langereis,
Holger Grüll, Chrit Moonen

http://www.sciencedirect.com/science/article/pii/S0168365912002593

 Thermosensitive liposomes made from 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1-stearoyl-
2-hydroxy-sn-glycero-3-phosphocholine (MSPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) were use to encapsulate TO-PRO-3, a cell-impermeable molecule that binds to nucleic acid and increases its fluorescence. Ultrasound treatment using microbubbles was used to help promote internalization of these nanoparticles, and heating to 47C helped to release the fluorophore in mice, as observed by fluorescence imaging.