Wednesday, October 24, 2012

A Smart Nanofiber Web That Captures and Releases Cells

Young-Jin Kim, Mitsuhiro Ebara, and Takao Aoyagi

Nanofibrous webs were made by electrospinning copolymer of NIPAAm and benzophenone, which can photocrosslink the nanofibers into a temperature-responsive web. Upon heating to 37C, the web is open for the cells (normal human dermal fibroblasts) and cooling to 4C, encapsulates the cells. Re-heating to 37C releases the trapped cells, and this cooling and re-heating process can be repeated to release more cells. A live/dead assay and MTT viabiltiy assay shows the cells are living and can proliferate.

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