Wednesday, October 24, 2012

Genetically Encoded Cyclopropene Directs Rapid, Photoclick- Chemistry-Mediated Protein Labeling in Mammalian Cells

Zhipeng Yu, Yanchao Pan, Zhiyong Wang, Jiangyun Wang, and Qing Lin

The authors synthesized a stable cyclopropene based amino acid that they were able to site-specifically incorporate into E.coli and mammalian cell proteins and label by clicking with a tetrazole-based fluorophore. They characterized its reactivity,  site-selective incorporation, and selective labelleling.

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